Bruno Batista Bortoluzzi, Caciara Gonzatto Maciel, Antônio Rosa de Sousa Neto, Daniela Reis Joaquim de Freitas


Introduction: Although respiratory diseases have different etiologies, many of them are related to ambient air pollution. Breathing polluted air, fine particles can be inhaled, which contain pathogens that penetrate the lungs and the cardiovascular system causing infections. The objective of this study was to quantify and identify fungal bioaerosols in three different environments of a private university in Santa Catarina state. Outline: The samples were collected at October 7, 2016, in the canteen, library and classroom, with two periods of exposure: 30 minutes and two hours, in three periods of the day (morning, afternoon and evening). Colony forming units counting was performed, as well as observation of micro and macromorphological characteristics for identification of genera of fungi. Results: It was found that microbiological contamination is directly related to the flow of air and people circulating in closed environments. The genera of fungi most prevalent were Gliocladium, Fusarium, Penicillium and Cladosporium. Implications: The fungi identified in the study can cause problems for human health, requiring special attention to the microbiological control of the air, especially in indoor environments where the flow of people is high and there is not adequate air exchange.


Microbiologia do Ar; Fungos; Ar Condicionado.

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WHO. World Health Organization. The top 10 causes of death. Geneva: WHO; 2018. Available from:

WHO. World Health Organization. 9 out of 10 people worldwide breathe polluted air, but more countries are taking action. Geneva:

WHO; 2018. Available from:

WHO. World Health Organization. CLEAN AIR FOR HEALTH: Geneva Action Agenda. Geneva: WHO; 2018. Available from:

Trabulsi LR, Alterthum F. Microbiologia. 6. ed. São Paulo: Atheneu; 2016.

Alvarez-Moreno CA, Combariza JF. Risk of invasive fungal infections during hospital construction: how to minimize its impact in immunocompromised patients. Curr Opin Infect Dis [Internet]. 2019 Aug [cited 2019 Jul 10]; 32(4):322–329. Available from:

Ng SMS, Yap JM, Lau QY, Ng FM, Ong EHQ, Barkham T, et al. Brian Chia CS. Structure-activity relationship studies of ultra-short peptides with potent activities against fluconazole-resistant Candida albicans. Eur J Med Chem [Internet]. 2018 Apr [cited 2019 Jul 10]; 150:479–490. Available from:

Holgate ST. 'Every breath we take: the lifelong impact of air pollution' – a call for action. Clin Med (Lond) [Internet]. 2017 Feb [cited 2019 Jul 10]; 17(1):8–12. Available from:

Dehghani M, Sorooshian A, Nazmara S, Baghani AN, Delikhoon M. Concentration and type of bioaerosols before and after conventional disinfection and sterilization procedures inside hospital operating rooms. Ecotoxicol Environ Saf [Internet]. 2018 Nov

[cited 2019 Jul 10]; 164:277–282. Available from:

Cardoso ALSP, Tessari ENC, Kanashiro AMI, Stoppa GFZ, Luciano RL, Castro AGM. Avaliação da qualidade sanitária de incubatório por meio de placas de sedimentação. Arq Inst Biol [Internet]. 2009 Jun [cited 2019 Jul 10]; 76(2):279–283. Available from:

Salomon GM, Hjelmroos-Koski M, Rotkin-Ellman M.; Hammond S. K. Airborne mold and endotoxin concentrations in New Orleans, Louisiana, after flooding, October through November 2005. Environ Health Perspect. [Internet]. 2006 Sep [cited 2019 Jul 10];

(9):1381–1386. Available from:

Barnet HL, Hunter BB. Illustred Genera of Imperfect Fungi. 4. ed. Minnesota: Burgess Publishing Company; 1972.

Hoog GS, Guarro J, Gené J, Figueiras MJ. Atlas Of Clinical Fungi. 2. ed. Washington: Amer Society for Microbiology; 2001.

Kern MEE, Blevins KS. Micologia Médica. 2.ed. São Paulo: Premier; 1999.

Friberg B, Friberg S, Burman LG. Correlation between surface and air counts of particles carrying aerobic bacteria in operating rooms with turbulent ventilation: an experimental study. J Hosp Infect [Internet]. 1999 May [cited 2019 Jul 10]; 42(1):61–68. Available from:

Pantoja LDM. Estimativa Dos Níveis De Bioaerossóis e Compostos Orgânicos Voláteis Fúngicos em Ambientes Ocupacionais No Município De Fortaleza-Ceará. [Tese de Doutorado]. Fortaleza: Universidade Federal do Ceará, Pós-Graduação em Engenharia Civil; 2016. Available from:

Agência Nacional de Vigilância Sanitária. Resolução-RE nº 09, de 16 de janeiro de 2003. Determina a publicação de Orientação Técnica elaborada por Grupo Técnico Assessor, sobre Padrões Referenciais de Qualidade do Ar Interior, em ambientes climatizados artificialmente de uso público e coletivo. Diário Oficial da União, Brasília, DF, 20 jan 2006. Available from:

Barreiros G, Akiti T, Magalhães AC, Nouér SA, Nucci M. Effect of the implosion and demolition of a hospital building on the concentration of fungi in the air. Mycoses [Internet]. 2015 Dec [cited 2019 Jul 10]; 58(12):707–713. Available from:

Cacia de Melo Machado E, Cezar Limberger V, de Cassia de Souza Schneider R, Corbellini VA. Evaluation of the quality of air in a surgical center of a hospital in the south of Brazil. Rev Salud Publica (Bogota) [Internet]. 2016 Jun [cited 2019 Jul 10]; 18(3):447–458. Available from:

Cho SY, Myong JP, Kim WB, Park C, Lee SJ, Lee SH, Lee DG. Profiles of Environmental Mold: Indoor and Outdoor Air Sampling in a Hematology Hospital in Seoul, South Korea. Int J Environ Res Public Health [Internet]. 2018 Nov 15 [cited 2019 Jul 10]; 15(11):112. Available from:

Sobral LV, Melo KN, Souza CM, Silva SF, Silva GLR, Silva ALF, et al. Antimicrobial and enzymatic activity of anemophilous fungi of a public university in Brazil. An. Acad. Bras. Ciênc [Internet]. 2017 Oct [cited 2019 Jul 10]; 89(3):2327–2340. Available from:

Bergadia FEL, Laachari F, Sadiki M, Elabed S, Iraqui MH, Ibnsouda SK. Determination of endoglucanase activity of paper decaying fungi from an old library at the ancient Medina of Fez. Microbiology [Internet]. 2016 Feb [cited 2019 Jul 10]; 85(1)47–55. Available from:

Polo A, Cappitelli F, Villa F, Pinzari F. Biological invasion in the indoor environment: the spread of Eurotium halophilicum on library materials. Internat Biodeter Biodegradation [Internet]. 2017 Mar [cited 2019 Jul 10]; 118:34–44. Available from:

Osimani A, Garofalo C, Milanović V, Taccari M, Aquilanti L, Polverigiani S, et al. Indoor air quality in mass catering plants: Occurrence of airborne eumycetes in a university canteen. Internat J Hospit Management [Internet]. 2016 Oct [cited 2019 Jul 10]; 59:1–10. Available from:

Asif A., Zeeshan M, Jahanzaib M. Assessment of indoor and outdoor microbial air quality of cafeterias of an educational institute. Atmospheric Pollution Research [Internet]. 2019 Mar [cited 2019 Jul 10]; 10(2)531–536. Available from:

Rodrigues KS. Ocorrência de fungos com potencial toxígeno em castanha-do-brasil no estado de Roraima. [Tese de Mestrado]. Boa Vista: Universidade Estadual de Roraima, Pós-Graduação em Agroecologia; 2016. Available from:

Lee HJ, Ryu D. Worldwide Occurrence of Mycotoxins in Cereals and Cereal-Derived Food Products: Public Health Perspectives of Their Co-occurrence. J Agric Food Chem [Internet]. 2017 Aug [cited 2019 Jul 10]; 65(33):7034–7051. Available from:

Pitt JI, Miller JD. A Concise History of Mycotoxin Research. J Agric Food Chem [Internet]. 2017 Aug [cited 2019 Jul 10]; 65(33):7021–7033. Available from:

Gruber-Dorninger C, Novak B, Nagl V, Berthiller F. Emerging Mycotoxins: BeyondTraditionally Determined Food Contaminants. J Agric Food Chem [Internet]. 2017 Aug [cited 2019 Jul 10]; 65(33):7052–7070. Available from:

Souza JMO, Carneiro MFH, Paulelli ACC, Grotto D, Júnior AMM, Júnior FB, et al. Arsenic and rice: toxicity, metabolism, and food safety. Química Nova [Internet]. 2014 Oct [cited 2019 Jul 10]; 38(1): 118–127. Available from:–40422015000100118

Santana MS, Santana LS, Silva OMC, Ximenes LMAS. Incidência de doenças fúngicas no maracujazeiro (passiflora edulis sp.) em propriedades familiares no município de Alta Floresta – MT. Enciclopédia Biosfera [Internet]. 2018. Jul [cited 2019 Jul 10]; 15(27):66–81. Available from:

Jara D, Portnoy J, Dhar M, Barnes C. Relation of indoor and outdoor airborne fungal spore levels in the Kansas City metropolitan area. Allergy Asthma Proc [Internet]. 2017 Mar [cited 2019 jul 10]; 38(2):130–135. Available from:

Ziaee A, Zia M, Goli M. Identification of saprophytic and allergenic fungi in indoor and outdoor environments. Environ Monit Assess [Internet]. 2018 Sep [cited 2019 Jul 10]; 190(10):574. Available from:

Crawford JA, Rosenbaum PF, Anagnost SE, Hunt A, Abraham JL. Indicators of airborne fungal concentrations in urban homes: understanding the conditions that affect indoor fungal exposures. Sci Total Environ [Internet]. 2015 Jun [cited 2019 Jul 10]; 517:113–24. Available from:

Tupaki-Sreepurna A, Kindo AJ. Fusarium: The versatile pathogen. Indian J Med Microbiol [Internet]. 2018 Maio [cited 2019 Jul 10]; 36(1):8–17. Available from:;year=2018;volume=36;issue=1;spage=8;epage=17;aulast=Tupaki%2DSreepurna

Ferrándiz-Pulido C, Martin-Gomez MT, Repiso T, Juárez-Dobjanschi C, Ferrer B, López-Lerma I, et al. Cutaneous infections by dematiaceous opportunistic fungi: Diagnosis and management in 11 solid organ transplant recipients. Mycoses [Internet]. 2019 Feb [cited 2019 Jul 10]; 62(2):121–127. Available from:

Grava S, Lopes FA, Cavallazzi RS, Grassi MF, Svidzinski TI. A rare case of hemorrhagic pneumonia due to Cladosporium cladosporioides. J Bras Pneumol [Internet]. 2016 Oct [cited 2019 Jul 10]; 42(5):392–394. Available from:

Arikoglu T, Batmaz SB, Coşkun T, Otag F, Yildirim DD, Kuyucu S. The characteristics of indoor and outdoor fungi and their relation with allergic respiratory diseases in the southern region of Turkey. Environ Monit Assess [Internet]. 2016 Jun [cited 2019 Jul 10];

(6):380. Available from:

Venkatesh R, Gurav P, Agarwal M, Sapra N, Dave PA. Ocular infection with Gliocladium species-report of a case. J Ophthalmic Inflamm Infect [Internet]. 2017 Dec [cited 2019 Jul 10]; 7(1):9. Available from:

Chan JF, Lau SK, Yuen KY, Woo PC. Talaromyces (Penicillium) marneffei infection in non-HIV-infected patients. Emerg Microbes Infect [Internet]. 2016 Mar [cited 2019 Jul 10]; 5:e19. Available from:

Sood G, Huber K, Dam L, Riedel S, Grubb L, Zenilman J, et al. Pseudo-outbreak of Penicillium in an outpatient obstetrics and gynecology clinic. Am J Infect Control [Internet]. 2017 May [cited 2019 Jul 10]; 45(5):557–558. Available from:

Kauffmann-Lacroix C, Costa D, Imbert C. Fungi, Water Supply and Biofilms. Adv Exp Med Biol [Internet]. 2016 May [cited 2019 Jul 10]; 931:49–61. Available from:

Patel TY, Buttner M, Rivas D, Cross C, Bazylinski DA, Seggev J. Variation in airborne fungal spore concentrations among five monitoring locations in a desert urban environment. Environ Monit Assess [Internet]. 2018 Oct [cited 2019 Jul 10]; 190(11):634.

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